The Humboldt Forum (proper spelling Humboldt Forum) in the Berlin Palace is a universal museum in the Mitte district of Berlin. In addition to the Ethnological Museum and the Museum of Asian Art of the Berlin State Museums, it is also home to the Berlin exhibition of the Berlin City Museum and the Humboldt Laboratory of the Humboldt University of Berlin. In addition, accompanying events, exhibitions and guided tours take place in the facility supported by the Humboldt Forum Foundation in the Berlin Palace.

The Mühlendammschleuse in Berlin is located in the Mitte district east of Mühlendamm on the Fischerinsel. It lies in the course of the Spree, which is part of the Spree-Oder waterway here. In the early days, a fut v connected the towns of Berlin and Cölln, from which the city of Berlin later grew. This is where Berlin was founded. The lock was put into operation in 1942 and overcomes a drop of 1.51 metres

The Nikolaiviertel in Berlin's Mitte district is the oldest settlement area in the capital. Almost completely destroyed in World War II, it was rebuilt in 1980-1987 by architect Günter Stahn on behalf of the East Berlin magistrate on the occasion of the city's 750th anniversary. Around the reconstructed Nikolaikirche, an ensemble of historic town houses and correspondingly adapted prefabricated buildings was built on an almost medieval floor plan. The monument is now one of the sights of Berlin.

At 368 metres, the Berlin television tower is the tallest building in Germany and the fifth tallest television tower in Europe. The television tower is located in the park at the television tower in Berlin's Mitte district. When it was completed in 1969, it was the second highest television tower in the world and, with over a million visitors a year, is one of the ten most popular sights in Germany.

The Museum Island is a building ensemble consisting of five museums in the northern part of the Spreeinsel in the historic centre of Berlin. It is one of the most important sights in the German capital and one of the most important museum complexes in Europe. Built between 1830 and 1930 on behalf of the Prussian kings according to plans by five architects, it was included in the UNESCO World Heritage List as a whole in 1999. The Museum Island consists of the Old Museum, the New Museum, the Old National Gallery, the Bode Museum and the Pergamon Museum. Since the reunification of Germany, it has been renovated and expanded as part of the Museum Island master plan. On July 12, 2019, the James Simon Gallery opened as a new visitor centre. Also in the northern part of the Spreeinsel is the Berlin Cathedral at the Lustgarten, as well as the Humboldt Forum, which is used for museums and culture and was built in the form of the Berlin Palace by 2021.

The Reichstag building (in short: Reichstag; officially: plenary area Reichstag building; unofficially also Bundestag or Wallot-Bau) on the Republic Square in Berlin has been the seat of the German Bundestag since 1999. The building was erected between 1884 and 1894 in Neo-Renaissance style according to plans by the architect Paul Wallot in the Tiergarten district on the left bank of the Spree. It housed both the Reichstag of the German Empire and that of the Weimar Republic. Initially, the Federal Council of the Empire also met there. After severe damage from the Reichstag fire of 1933 and World War II, the building was restored in a modernised form in the 1960s. From 1995 to 1999, the Reichstag was fundamentally redesigned by Norman Foster for permanent use as a parliament building, which was decided in 1991. The German Bundestag meets here. A landmark in the cityscape is the accessible glass dome above the plenary hall based on an idea by Gottfried Böhm.

Berlin Hauptbahnhof is the most important passenger station in Berlin and the largest tower station in Europe. With around 330,000 travellers and visitors every day, it is the fourth most frequented long-distance train station of Deutsche Bahn after Hamburg, Frankfurt (Main) and Munich. The crossing station with 14 platform tracks is a transfer point between long-distance and local passenger transport. Around 1300 trains stop here every day. There are other public transport connections in front of the station with the inner-city bus service (also metro and night buses) and the BVG tram (lines M5, M8 and M10). There is also a subway station of the same name (U5). The striking building was designed by architect Meinhard von Gerkan. Together with the station, a new north-south railway line was put into operation on May 28, 2006 through the tunnel north-south long-distance railway, which realised a complete conversion and reorganization of rail passenger transport in Berlin through the so-called "mushroom concept".

The Federal Chancellery is a supreme federal authority that supports the German Federal Chancellor in his duties. It has its headquarters or first office in the federal capital of Berlin and its second office in the federal city of Bonn. Wolfgang Schmidt (SPD) is head of the Federal Chancellery and Federal Minister for Special Tasks. The Chancellery currently employs around 600 people (as of 2019). Since 2001, a new building designed by Axel Schultes in Berlin's Spreebogen, separated from the Reichstag building by Platz der Republik, has served as the seat of the authorities. The building opens onto a central square opposite the Paul-Löbe-Haus, which is to be designed as a citizens' forum. It is part of the "Bund des Bunds" group of buildings at the Spreebogenpark. Employees of the Minister of State for Culture are also currently working in the building.

The House of World Cultures (HKW) is an exhibition space in Berlin for international contemporary art and a forum for current developments and discourses. It presents artistic productions from all over the world with a special focus on non-European cultures and societies. Since it was founded in March 1989, the House of World Cultures has been based in the former congress hall on the banks of the Spree in the Tiergarten and government district. As an icon of architectural modernism, the Congress Hall became a prominent symbol of the German-American alliance. In the vernacular of Berlin, the building is also known under the name "Pregnant Oyster" based on its shape.