Your experience begins as you pass by five historic train cars to enter the museum through the gift shop. Inside the gift shop, staff and tour guides will be waiting to tell you about the day's tour schedule and assist with anything you may need. The gift shop offers all kinds of train memorabilia, toy trains, puzzles, and anything else a train fan could want, as well as themed activities.

The museum has something for all ages. Vintage equipment and artefacts will stoke the memories of those old enough to have experienced this bygone era in the United States' transport past. Children will be both entertained and educated by informative, interactive displays. Hear from a real L&N cook, Pullman porter, and conductor. See what life was like for these employees as well as for the travellers.

Stroll through the museum at your own pace until it's time for your guided tour. Your guide will meet you on the ground floor of the museum on the same wooden benches used for passengers in the depot since 1925. As you sit inside, hear about the history of the Louisville and Nashville railway and more. In the next portion of your tour, you'll get to board the train cars, starting in an E8 diesel-electric locomotive.

Then you'll move into the Railway Post Office, or RPO car. Next have a seat in the Duncan Hines Dining Car, and find out what meals were like for those eating, cooking, and serving on the trains. See what it was like to travel in first, second, and third class aboard the Towering Pine Sleeper Car. The last stop on your tour is the L&N Presidential Car, the oldest surviving passenger car from the L&N Railway.